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| University of Michigan > Medical System > Center for Gene Therapy > The Vector Core |
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Note: As of 9/19/01, the NGVL plasmids have been renamed as UMVC plasmids to reflect the completion of the NGVL program at the University of Michigan. The plasmids are still available to all investigators at the same price, but the reference name has changed. If you have any questions please contact the lab supervisor at lanigant@umich.edu .
| Viral Vector | Maximum Insert Size | Preferential Target Cells | Common Research Applications | General Advantages |
| Adenovirus | 7.5 kb | Transduces cells with CAR & avB integrinsreceptors very well
Will transiently transduce many primary cells at high effeciency |
In vitro and in vivo transgene expression
siRNA |
Ease of use in vitro and in vivo
High titiers |
| Adeno-Associated Virus (AAV) | 3.5 kb | Transfects wide variety of cell types, including muscle, nervous, liver, skin, and eye | Non-immunogenic and thus practical for in
vivo applications Transgene expression has been shown to persist for over a year Been used successfully in gene therapy trials for hemophilia in pre-clinical animal studies |
No viral genes present or expressed Non-pathogenic to humans Can deliver transgenes to dividing and non-dividing cells Long-term transgene expression |
| Lentivirus (FIV) | > 8 kb | Will transduce (via integration) differentiated non-dividing cells including Hematopoietic and Nervous, as well as dividing cells such as liver and skin | Stable integration allows for both production of permanent cell lines for in vitro applications and long-term modifications for in vivo and ex vivo applications |
Transfects dividing and non-dividing cells Integration into genome Wide spectrum of target cells |
| Retrovirus (MoMLV) | > 8 kb | Transfects and integrates into wide variety of dividing cells | Stable integration allows for both production of permanent cell lines for in vitro applications and long-term modifications for in vivo and ex vivo applications |
Integration into genome Wide spectrum of target cells |